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vc-02 pancreatic endoderm cells (pec-01) in macroencapsulation device hesc cyt49 allo – t1dm  (ViaCyte Inc)

 
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    ViaCyte Inc vc-02 pancreatic endoderm cells (pec-01) in macroencapsulation device hesc cyt49 allo – t1dm
    Vc 02 Pancreatic Endoderm Cells (Pec 01) In Macroencapsulation Device Hesc Cyt49 Allo – T1dm, supplied by ViaCyte Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyt49+hescs/pec+direct/pm39753110-63-55-66
    Average 90 stars, based on 1 article reviews
    vc-02 pancreatic endoderm cells (pec-01) in macroencapsulation device hesc cyt49 allo – t1dm - by Bioz Stars, 2026-09
    90/100 stars

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    Plasmid Preparation:

    Article Title: Universal donor cells
    Article Snippet: .. CyT49 hESCs (ViaCyte) were electroporated with an RNP complex comprising B2M-2 gRNA (SEQ ID NO: 2) and Cas9 protein in molar ratio of 3:1 (gRNA:Cas9) and 4 μg of the B2M-CAGGS-PD-L1 donor plasmid (SEQ ID NO: 33) for 2 pulses of 30 ms at 1100 V. Following electroporation, the cells were pipetted out into an Eppendorf tube filled with StemFlex media with RevitaCell. ..

    Article Title: Universal donor cells
    Article Snippet: .. CyT49 hESCs (ViaCyte) were electroporated with an RNP complex comprising B2M-2 gRNA (SEQ ID NO: 2) and Cas9 protein in molar ratio of 3:1 (gRNA:Cas9) and 4 μg of the B2M-CAGGS-PD-L1 donor plasmid (SEQ ID NO: 33) for 2 pulses of 30 ms at 1100 V. Following electroporation, the cells were pipetted out into an Eppendorf tube filled with StemFlex media with RevitaCell. .. This cell suspension was then plated into tissue culture dishes pre-coated with BIOLAMININ 521 CTG at 1:20 dilution.

    Electroporation:

    Article Title: Universal donor cells
    Article Snippet: .. CyT49 hESCs (ViaCyte) were electroporated with an RNP complex comprising B2M-2 gRNA (SEQ ID NO: 2) and Cas9 protein in molar ratio of 3:1 (gRNA:Cas9) and 4 μg of the B2M-CAGGS-PD-L1 donor plasmid (SEQ ID NO: 33) for 2 pulses of 30 ms at 1100 V. Following electroporation, the cells were pipetted out into an Eppendorf tube filled with StemFlex media with RevitaCell. ..

    Article Title: Universal donor cells
    Article Snippet: .. Using B2M-2 gRNA, CyT49 hESCs (ViaCyte) were electroporated and single-cell sorted 3 days post electroporation using the WOLF FACS-sorter (Nanocellect) into BIOLAMININ 521 CTG coated 96-well plates with StemFlex and Revitacell. ..

    Article Title: Universal donor cells
    Article Snippet: .. CyT49 hESCs (ViaCyte) were electroporated with an RNP complex comprising B2M-2 gRNA (SEQ ID NO: 2) and Cas9 protein in molar ratio of 3:1 (gRNA:Cas9) and 4 μg of the B2M-CAGGS-PD-L1 donor plasmid (SEQ ID NO: 33) for 2 pulses of 30 ms at 1100 V. Following electroporation, the cells were pipetted out into an Eppendorf tube filled with StemFlex media with RevitaCell. .. This cell suspension was then plated into tissue culture dishes pre-coated with BIOLAMININ 521 CTG at 1:20 dilution.

    Article Title: Universal donor cells
    Article Snippet: .. Using B2M-2 gRNA, CyT49 hESCs (ViaCyte) were electroporated and single-cell sorted 3 days post electroporation using the WOLF FACS-sorter (Nanocellect) into BIOLAMININ 521 CTG coated 96-well plates with StemFlex and Revitacell. ..

    Single Cell:

    Article Title: Universal donor cells
    Article Snippet: .. Using B2M-2 gRNA, CyT49 hESCs (ViaCyte) were electroporated and single-cell sorted 3 days post electroporation using the WOLF FACS-sorter (Nanocellect) into BIOLAMININ 521 CTG coated 96-well plates with StemFlex and Revitacell. ..

    Article Title: Universal donor cells
    Article Snippet: .. Using B2M-2 gRNA, CyT49 hESCs (ViaCyte) were electroporated and single-cell sorted 3 days post electroporation using the WOLF FACS-sorter (Nanocellect) into BIOLAMININ 521 CTG coated 96-well plates with StemFlex and Revitacell. ..

    FACS:

    Article Title: Universal donor cells
    Article Snippet: .. Using B2M-2 gRNA, CyT49 hESCs (ViaCyte) were electroporated and single-cell sorted 3 days post electroporation using the WOLF FACS-sorter (Nanocellect) into BIOLAMININ 521 CTG coated 96-well plates with StemFlex and Revitacell. ..

    Article Title: Universal donor cells
    Article Snippet: .. Using B2M-2 gRNA, CyT49 hESCs (ViaCyte) were electroporated and single-cell sorted 3 days post electroporation using the WOLF FACS-sorter (Nanocellect) into BIOLAMININ 521 CTG coated 96-well plates with StemFlex and Revitacell. ..



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: Insulin expression and C-peptide in type 1 diabetes subjects implanted with stem cell-derived pancreatic endoderm cells in an encapsulation device

    doi: 10.1016/j.xcrm.2021.100466

    Figure Lengend Snippet:

    Article Snippet: The CyT49 hESC line was derived by ViaCyte from an ethically-consented embryo in a process compliant with governing regulations and is the starting material for cell product manufacture.

    Techniques: Software